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Full Automation From Library Samples to Assay Plates with “Calli-L”
07.10.2009

Innovative Automation with CALLI - L

The sampling of valuable material from compound libraries with subsequent dissolution to a set concentration for biological screening has always been seen as a process that is complicated and difficult to automate without human intervention.

“Calli-L” from Zinsser Analytic introduces an innovative approach to fully automate this tedious workflow: The distribution of solid library samples, controlled dissolution and the final preparation of assay plates for biological assays. Depending on the required throughput, all 3 steps can be either set up on a single workbench or on 2 or 3 integrated workbench systems.

“Calli-L” uses new powder dispensing technology “Redi2plus”, that reads and compares the sample barcode with that stored in the “in-house” database, extracts the required distribution data, decaps the library vial prior to solid sampling and dispenses the resulting sample into the dissolution vial. The database is subsequently updated with the final weight of the library and dissolution vial before both are re-capped. Trials show that about 60% of all library samples can be distributed with this system. Sensitive samples are processed under an argon shield.

Dissolution of the solid samples under an inert atmosphere is the next stage. 8 independent probes are used to add DMSO or other solvents simultaneously with argon, at a volume to provide a set sample concentration. Batches of 96,192 or 384 samples are dissolved using special vortexers under an argon shield. A dissolution check decides whether any additional sample treatment is necessary e.g. sonication. When the dissolution is complete, the samples are dispensed under argon into assay-, mother- and storage plates.

“Calli-L” can be customised to fit your dissolution needs exactly. Up to 384 samples can be processed per hour depending on instrument configuration.

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