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FISH: Automated Fluorescence In-Situ Hybridisation

Task

FISH (Fluorescence In-Situ Hybridisation) is a technique used in the diagnosis of leukaemia to determine the disease phenotype, eg AML M1, M2 AML.

Blood cells are grown in a short-term cell culture and a sample as a film is examined under a microscope to locate and identify areas exhibiting metaphase nuclei. Leukaemia type-specific probes are then pipetted onto these areas and a coverslip applied. After sealing of the coverslips with natural rubber, the treated slides are then incubated in a hybridization oven and the fluorescence data obtained.

 

Solution

"LISSY FISH" automates the application of the FISH type-specific leukaemia probes onto designated areas of the slides and applies the coverslips. The slides are barcoded and this identifier, plus the XY coordinates of the areas to be xamined, the identifier for the type-specific FISH probe and the volume to be applied are stored as a work list in the site LIMS system. Up to 24 slides together with the FISH probes can be located on the workbench of the system in a run. A bar code camera reads the barcode identifier of both the slide and the FISH probes and cross references these against the data in the work list from the LIMS system with any mismatches being flagged. If the volume for a required FISH probe as determined by the work list is not sufficient for a run, a warning is displayed.

"LISSY FISH" applies the FISH probes to the designated areas of the slides in a multi-pipetting cycle and immediately applies the coverslip. When all slides have been processed, they are taken from the system and manually sealed with natural rubber and the system is now ready for the next 24 slide run. "LISSY FISH" has been developed for research use only. It is not suitable for use in diagnostic procedures.